SAR-CoV-2 embedded in human cells, IgG4, Autoimmune Diseases and Cancer

This article discusses the study that showed that SARS-CoV-2 mRNA can be changed to DNA, making the body produce SARS-CoV-2 proteins.[1][2]

Long-lasting exposure to human-produced SARS-CoV-2 proteins affects the immune system, as evident by the increase in IgG4, and makes the body more vulnerable to cancer and autoimmune development.

With that summary, let’s proceed with the details and the studies that support each.

SARS-CoV-2 mRNA to DNA

Classically, DNA is transcribed to become RNA, then RNA is translated to make proteins.

The idea behind the mRNA COVID-19 shots is to make the body produce the SARS-CoV-2 spike proteins, the part of the virus that sticks out to attach to human cells.

Spike proteins from the COVID shots are then recognized by the immune system and destroyed. In that brief encounter, the immune system records the spike protein and makes immune cells against it which can be produced rapidly on future exposures, which is how immunity develops.

The faster immune response on subsequent exposure prevents the increase of SARS-CoV-2, which can overwhelm the body and lead to severe COVID-19 and possibly death.

However, RNA can become DNA by a process called reverse transcription.

A study by Zhang et al. Reverse-transcribed SARS-CoV-2 RNA can integrate into the genome of cultured human cells and can be expressed in patient-derived tissues; published by the Proceedings of the National Academy of Sciences in May 2021 demonstrated that the RNA of the round, the central part of the SARS-CoV-2 called the nucleocapsid, can be reverse-transcribed into DNA. [2]

The study was well-defended against comments, and I discussed that in my article, SARS-CoV-2 RNA Reverse Transcribed to Human DNA

Even though they used the nucleocapsid instead of the spike protein in their research, the results should probably be the same if they used the RNA strands of the SARS-CoV-2. I don’t think the reverse transcription enzymes discriminate between the two. (Please correct me if I’m wrong and cite references.)

Using the nucleocapsid strand for the study made the paper’s publication easier. It would have attracted too much attention and gained notoriety in 2021 if the study showed that the spike protein mRNA could become DNA and integrate into human cells. I credit the authors for that intelligent move.

In February this year, six of the eight authors of the same study published LINE1-Mediated Reverse Transcription and Genomic Integration of SARS-CoV-2 mRNA Detected in Virus-Infected but Not in Viral mRNA-Transfected Cells. Another prestigious journal, Viruses, published it.[2]

This time, their results showed that the SARS-CoV-2 RNA quickly becomes DNA in cells infected with SARS-CoV-2 by a thousandfold compared to uninfected cells.

Infected cells are stressed and produce Long Interspersed Nuclear element-1 or LINE-1 excessively, an enzyme that changes RNA to DNA.

Nanopore WGS versus TagMap

The authors compared two methods of measuring LINE-1, Tag-Map, and Nanopore Whole Genome Sequencing).

They found that TagMap is more sensitive than Nanopore. “Tag-Map can be used to analyze a much larger number of cells (up to ~20,000 cells), which makes it a more sensitive way to detect rare retrotransposition events” (DNA⇒RNA).

This finding explains why the study by Smits et al., No evidence of human genome integration of SARS-CoV-2 found by long-read DNA sequencing., published in August 2021, did not detect any SARS-CoV-2 human cell integration. They used Nanopore sequencing.[4]

Can vaccine mRNA change to DNA?

The authors asked that question in their discussion.

An important question is whether vaccine mRNA can be subject to LINE1-mediated retrotransposition.[1]

The answer is it depends.

In uninfected cell line 293T, no SARS-CoV-2 DNA was found. However, in cells that overexpressed LINE1, SARS-CoV-2 retrotransposed RNA (DNA) increased 1000 times!

As a positive control, we detected retrotransposed transfected viral nucleocapsid mRNA in cells that overexpressed LINE1 (which, as noted above, should increase retrotransposition by about 1000×), although the retrotransposed copies were ~10 times lower than that in virus-infected cells.

Viral RNA and LINE-1 were higher in COVID-19-infected cells. That’s because of large amounts of SARS-CoV-2 and LINE-1 during infections. Additionally, COVID-19 illness prevents the inhibition of retrotransposition by the immune system.

There are several possible explanations for the differences in the levels of retrotransposition in infected and transfected cells:

(i) The relative abundance of viral RNA is almost two orders of magnitude higher in infected cells than in transfected cells, which would increase the probability of association with LINE1 proteins;

(ii) virus infection, but not viral mRNA transfection, can induce endogenous LINE1 expression;

(iii) multiple factors during SARS-CoV-2 infection can inhibit the antiviral/anti-retrotransposition function of stress granules, which could increase retrotransposition.[1]

Evidence of Spike Proteins in Human Tissue

Dr. Arne Burkhardt presented a series of autopsies at Sweden’s Pandemic Strategies Lessons and Consequences conference early this year.

He identified spike proteins in the prostate, blood vessels, and muscles in several COVID-vaccinated corpses.

New and Alarming Autopsy Findings after the COVID shots

The image below from Dr. Burkhardt’s presentation shows spike proteins (dark brown) in the prostate gland.

Significance

The study explains why older adults and with comorbidities have higher COVID-19 risk

The following conditions produce more LINE-1; elderly adults[4], immune-compromised [5], presence of neuropsychiatric, autoimmune diseases, and various forms of cancer.[6]

The presence of more SARS-CoV-2 during infection and higher amounts of LINE-1 in these populations can lead to more SARS-CoV-2 DNA embedded in human organs.

The antibodies against the foreign SARS-CoV-2 proteins can then attack the viruses and the cells with embedded foreign proteins.

The result is inflammation of several organs, including the blood vessels, heart, lungs, kidneys, and other organs seen in severe COVID-19.

At the same time, autoimmune conditions were also seen. This was discussed in the review article Autoantibodies in COVID-19: frequency and Function.[7]

Another explanation for autoimmunity is molecular mimicry, where the protein sequences in the SARS-CoV-2 are similar to human proteins. This immune response against the virus can also attack human cells. See the related articles below.

IgG4: The Immune tolerant antibody

The consequence of having spike proteins present in human cells, even in small amounts, is that the immune system gets used to the SARS-CoV-2 protein and induces immune tolerance.

Typically, the immunoglobulins IgG1 and IgG3 are called upon in fighting infections and identifying and destroying newly formed cancer cells in the body.

IgG4 is an antibody that increases after allergy desensitization against substances that causes a severe allergic response. People who develop anaphylactic shock and life-threatening airway occlusion against peanut butter and bee stings are often recommended to have desensitization shots.

Allergy desensitization requires regular injections of a small amount of bee or peanut allergens to make the immune system “tolerant” to those allergens. The result is the prevention of a hyperactivated immune response if exposed again.

Chronic exposure in tiny amounts sounds like SARS-CoV-2 proteins in human cells after an injection.

Studies have shown that the Pfizer and Moderna mRNA shots increase the IgG4 against SARS-CoV-2. I discussed them in

More IgG4 in the body has severe consequences like COVID-19 reinfections, cancers, and autoimmune diseases. You can read about them in:

Autoimmune diseases

The presence of foreign proteins arising from SARS-CoV-2 DNA in human cells can contribute to the rise in autoimmune diseases following COVID-19 and its jab.[7],[8],[9]

Turbo Cancers

Dr. Ute Krüger presented several autopsies that showed turbo cancers among those who got the COVID shots. She gave her findings on Pandemic Strategies, Lessons, and Consequences.

I wrote about her findings, which include rapidly growing benign tumors, new and recurrent cancers, multiple primary cancers, and blood cancers,

Turbo Cancers after the COVID shots

These cancers could result from the disrupted immune response from the increased IgG4 after the shots.

Personal Note

I know four vaccinated people who died from accelerated cancer since the jabs were rolled out. Breast, throat, gallbladder, and esophagus cancer.

My one friend with esophageal cancer underwent surgery, radiation, and chemotherapy. She was doing well and making plans, but the cancer returned.

This time, it was turbocharged, and she passed away in weeks.

It should be noted that the study by Zhang et al. was done in different conditions, such that they used cell lines and did not use lipid nanoparticles. They recommend more studies to know more.

It probably won’t get done. The establishment does not grant funding to studies that they don’t want to know the results.

Truth heals. Lies kill. Don’t Get Sick!

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References:

Zhang L, Bisht P, Flamier A, Barrasa MI, Friesen M, Richards A, Hughes SH, Jaenisch R. LINE1-Mediated Reverse Transcription and Genomic Integration of SARS-CoV-2 mRNA Detected in Virus-Infected but Not in Viral mRNA-Transfected Cells. Viruses. 2023 Feb 25;15(3):629. doi: 10.3390/v15030629. PMID: 36992338; PMCID: PMC10057545.
Zhang L, Richards A, Barrasa MI, Hughes SH, Young RA, Jaenisch R. Reverse-transcribed SARS-CoV-2 RNA can integrate into the genome of cultured human cells and can be expressed in patient-derived tissues. Proc Natl Acad Sci U S A. 2021 May 25;118(21):e2105968118. doi: 10.1073/pnas.2105968118. PMID: 33958444; PMCID: PMC8166107.
Smits N, Rasmussen J, Bodea GO, Amarilla AA, Gerdes P, Sanchez-Luque FJ, Ajjikuttira P, Modhiran N, Liang B, Faivre J, Deveson IW, Khromykh AA, Watterson D, Ewing AD, Faulkner GJ. No evidence of human genome integration of SARS-CoV-2 found by long-read DNA sequencing. Cell Rep. 2021 Aug 17;36(7):109530. doi: 10.1016/j.celrep.2021.109530. Epub 2021 Jul 28. PMID: 34380018; PMCID: PMC8316065.
De Cecco M., Ito T., Petrashen A.P., Elias A.E., Skvir N.J., Criscione S.W., Caligiana A., Brocculi G., Adney E.M., Boeke J.D., et al. L1 drives IFN in senescent cells and promotes age-associated inflammation. Nature. 2019;566:73–78. doi: 10.1038/s41586-018-0784-9.
Jones R.B., Song H., Xu Y., Garrison K.E., Buzdin A.A., Anwar N., Hunter D.V., Mujib S., Mihajlovic V., Martin E., et al. LINE-1 retrotransposable element DNA accumulates in HIV-1-infected cells. J. Virol. 2013;87:13307–13320. doi: 10.1128/JVI.02257-13.
Protasova MS, Andreeva TV, Rogaev EI. Factors Regulating the Activity of LINE1 Retrotransposons. Genes (Basel). 2021 Sep 30;12(10):1562. doi: 10.3390/genes12101562. PMID: 34680956; PMCID: PMC8535693.
Gao ZW, Zhang HZ, Liu C, Dong K. Autoantibodies in COVID-19: frequency and function. Autoimmun Rev. 2021 Mar;20(3):102754. doi: 10.1016/j.autrev.2021.102754. Epub 2021 Jan 18. PMID: 33476817; PMCID: PMC7813482.
Watad A, De Marco G, Mahajna H, et al. Immune‐mediated disease flares or new‐onset disease in 27 subjects following mRNA/DNA SARS‐CoV‐2 vaccination. Vaccines. 2021;9(5):435.
Chen Y, Xu Z, Wang P, Li XM, Shuai ZW, Ye DQ, Pan HF. New-onset autoimmune phenomena post-COVID-19 vaccination. Immunology. 2022 Apr;165(4):386-401. doi: 10.1111/imm.13443. Epub 2022 Jan 7. PMID: 34957554.

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